contestada

You are in the lab trying to identify Protein X. The first gel you run for Protein X gives you one band at 30 kD and another band at 75 kD. You run another gel for Protein X using another technique. This time, you get one band at 210 kD. Circle below the possible techniques you used to obtain the above results.

Respuesta :

funkeyusuff52

Answer:

SDS plus reducing agent in the first gel & SDS alone in the second gel

Explanation:

  • SDS-PAGE separates proteins primarily by mass because the ionic detergent SDS denatures and binds to proteins to make them uniformly negatively charged.
  • Therefore when a current is applied, all SDS-bound proteins in a sample will move through the gel toward the positively charged electrode.
Ver imagen funkeyusuff52

Otras preguntas