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It is quite possible to have a positive reaction in a glucose carbohydrate broth and yet a negative reaction in a glucose use methyl red test. This is due to:
a. methyl red cannot detect the types of acid produced from glucose
b. phenol red can detect single acid production but methyl red requires more than one acid produced.
c. glucose makes acid in a Phenol red test but neutral products in a methyl red test
d. If amino acids are metabolized this will interfere with the methyl red reaction

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Answer:

Option B is correct.

Explanation:

Carbohydrate fermentation test; its main purpose is to know if a bacteria can ferment a specific carbohydrate or not. The test can be used for distinguishing bacterial species.

When fermentation of carbohydrate occurs, gaseous acids are produced. This production of the acid lower the pH of the medium undergoing the test, which can be detected by the color change of the pH indicator.

The Color changes only when sufficient amount of acid is produced. Phenol red can easily detect single acid production but METHYL RED NEED MORE ACID.

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