Which of the following statements regarding glycolysis is false?
A) Glycolysis is considered to be an ancient metabolic system because it does not require oxygen.
B) Glycolysis is considered to be an ancient metabolic system because it is not located in a membrane-bound organelle.
C) Glycolysis is considered to be an ancient metabolic system because it occurs universally.
D) Glycolysis is considered to be an ancient metabolic system because it is the most efficient metabolic pathway for ATP
synthesis.
E) Glycolysis is considered to be an ancient metabolic system because glucose is the universal substrate for glycolysis.

Glycolysis occurs in the cytoplasm of cells hence it occurs universally in most cells because all cells have a cytoplasm. Prokaryotic cells, the first cells before the emergence of eukaryotic cells, do not have mitochondria where the aerobic metabolism occurs. Therefore glycolysis came before the Citric cycle. Additionally, it is believed to be an ancient form of metabolism because it does not require oxygen. Primordial earth’s atmosphere did not have oxygen and was mainly composed of methane and carbon dioxide. Therefore aerobic metabolism would not have been possible (Krebs cycle utilizes oxygen) at the time.

Glycolysis is inefficient because it consumes two ATPs and produces 4 ATPs hence having a net of 2 ATPs. The cells later evolved a more efficient metabolic pathway which is Krebs cycle that produces a net of 34 ATPs. Organism evolves towards higher efficiency hence glycolysis must have preceded the Krebs cycle.

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