Answer:
you need 100ml of 5X TAE and 400ml of water.
Step-by-step explanation:
You need to use a rule of three:
[tex]C_1V_1=C_2V_2[/tex]
where:
[tex]\left \{ {{C_1= 5X} \atop {C_2=1X}} \right.[/tex]
and
[tex]\left \{ {{V_1 = V_{TAE}} \atop {V_2=500ml}} \right.[/tex]
Therefore:
[tex]V_{TAE} = \frac{V_2*C_2}{C_1}[/tex]
[tex]V_{TAE} = 100ml[/tex]
Then just rest the TAE volume to the final Volume and you get the amount of water that you need to reduce the concentration.
Answer:
Step-by-step explanation:
It shall be 100xl times the number of 1x tae
