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hydrolysis of decapeptide P with the enzyme trypsin affords the following fragments: Glu-Gly-Lys, Gln-Val-Ile, Ala-Ser-Phe-Lys. Hydrolysis with chymotrypsin yields: Lys-Gln-Val-Ile, Glu-Gly-Lys-Ala-Ser-Phe. What is the amino acid sequence of P?

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Answer:

The sequence of an amino acid P is:

Glu-Gly-Lys-Ala-Ser-Phe-Lys-Gln-Val-Ile

Explanation:

Fragments obtained on hydrolysis of decapeptide P by the action of an enzyme named trypsin:

  • Glu-Gly-Lys,
  • Gln-Val-Ile
  • Ala-Ser-Phe-Lys

Fragments obtained on hydrolysis of decapeptide P by the action of an enzyme named chymotrypsin:

  • Lys-Gln-Val-Ile,
  • Glu-Gly-Lys-Ala-Ser-Phe

In order to determine the sequence of protein P , we will arrange fragments in such a way so that common fragments or the common parts of fragments should come under each other.

On arranging these fragments :

Glu-Gly-Lys-Ala-Ser-Phe

Glu-Gly-Lys

                   Ala-Ser-Phe-Lys

                                         Lys-Gln-Val-Ile

                                                Gln-Val-Ile

The sequence of an amino acid P is:

Glu-Gly-Lys-Ala-Ser-Phe-Lys-Gln-Val-Ile

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