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Spontaneous generation or abiogenesis is the belief that living things could appear suddenly from non living matter without the need of a living progenitor to give life.This idea lasted for centuries until Pasteur's experiment proved otherwise. His experiments with flask's disapproved the idea and noted that micro-organisms are present in the air (atmosphere) and can contaminate solutions and that the air itself does not create microbes. He filled short necked flasks with broth (beef) and boiled the content, then left some of the flasks open in air to cool and others closed, he later found that those that were left opened were contaminated while the closed flasks were intact free of microorganisms. This observation enabled him to disprove the existing idea.

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