The molecular weight of hemoglobin can be calculated using osmotic pressure
Osmotic pressure is a colligative property and it depends on molarity as
πV = nRT
where
π = osmotic pressure
V = volume = 1mL = 0.001 L
n = moles
R = gas constant = 0.0821 L atm / mol K
T = temperature = 25°C = 25 + 273 K = 298 K
Putting values we will get value of moles
[tex]moles=\frac{\pi V}{RT}=\frac{0.00195X0.001}{0.0821X298}mol[/tex]
we know that
[tex]moles=\frac{mass}{molarmass}[/tex]
Therefore
[tex]molarmass=\frac{mass}{moles}=\frac{5.10X10^{-3}g}{7.97X10^{-8}}=6.399X10^{4}g[/tex]
