The correct answer is A) the removal of all gold from the Americas.
What was not a lasting impact of Spain had on the Americas was the removal of all gold from the Americas.
Although the Spanish wanted so much to take all the gold that they thought existed in some regions of the Americans, that never was possible. There were two legends that the Spanish heard that made them believe the existence of so much gold. One was the legend of the city of El Dorado, somewhere in Colombia, in the middle of the jungle. The other legend was that Aztec Emperor Montezuma had mines and bullions of gold that were someplace in the area of Tenochtitlan. Of course, that gold was never found.
What indeed was a lasting impact of Spain in the Americas was the prominence of the Spanish language, the large number of Catholics and the existence of animals such as horses.
