Given data:
Concentration of stock solution which is the initial solution = M1 = 1.00 * 10^3 mg/L
Concentration of prepared solution i.e final solution = M2 = 6.250 mg/L
Volume of final solution, V2 = 5.000 * 10^2 ml
To determine:
The initial volume i.e. volume of the stock solution required = V1
Calculation:
Use the relation
M1V1 = M2V2
V1 = M2V2/M1 = 6.250 mg.L-1 * 5.000*10^2 ml/1.00 * 10^3 mg.L-1
V1 = 3.125 *10^3 ml
The volume of stock solution required is 3.125 *10^3 ml