The enzyme catalysed reaction are affected by temperature, pH, and substrate concentration.
(1) The more substrate is, the more product will be until all the enzyme become saturated.
If we increase the amount to 20g the reaction rate will be equal to the 4g of substrate because now all the available enzyme are saturated. This known as Vmax.
(2) The optimum pH for this enzyme is 7 because in this pH the enzyme is most efficient. At pH 2, the enzyme might not able to work at all because this pH can disturb the enzyme structure.
(3) There is an optimum temperature for all enzyme. Increasing or decreasing this temperature affect the enzyme catalysed reaction. In fever the high body temperature affect the enzyme catalysed reaction in the body. This deactivate most of the enzyme and activate some specific enzymes.
Hence, we can conclude that the enzyme catalysed reaction are affected by temperature, pH, and substrate concentration.
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