Why was Rome's location significant?
Rome was located at the base of the Alps, which made it easy to trade with Gaul and Spain.
Rome was located on the eastern coast of the Italian peninsula where trade routes to Asia were developed.
Rome was located on the Mediterranean Sea, which made it easy to trade with several other civilizations.
Rome was located in the Apennine Mountain chain where trade routes to other civilizations were developed.

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Rome was located on the central coast of the Italian peninsula which then led trade routes to the Mediterranean Sea. Thus, it was much easier to trade with several other civilizations. Therefore, the correct answer is your THIRD choice: Rome was located on the Mediterranean Sea, which made it easy to trade with several other civilizations. 

Rome was located on the Mediterranean Sea, which made it easy to trade with several other civilizations.