I conducted a Bradford assay and included three samples and collected the absorbances of each. One undiluted, one in a 1:2 dilution, and one in a 1:7 dilution. Now that I'm calculating the concentration:
a. What is the purpose of diluting the samples?
b. How does dilution affect the absorbance readings?
c. How do you calculate the concentration of the original sample from the absorbance readings?
d. Why is a standard curve necessary for accurate concentration determination in a Bradford assay?