You work in a lab and are given two cultures A and B. Culture A is labeled as an obligate anaerobe that is psychrophilic and gram negative. Culture B is labeled as an obligate anaerobe that is mesophilic and gram positive. You inoculate each culture onto a general purpose agar plate. After incubation, you observe the two plates and see no growth on either one. You know you messed up and need to troubleshoot. Which of the following describes what you should have done?
1) Both cultures should have been placed in high oxygen environments but with different pH's.
2) They both would have grown if you had placed them in anaerobic jars.
3) They both should be at different pH's and different temperatures.
4) They both should have received a pre-treatment of 90 degrees celsius before placing in an anaerobic chamber.
5) They both should have been placed in anaerobic jars - culture A at about 15 degrees celsius and culture B at 37 degrees celsius.
6) They both needed to be placed on selective media and then they would have grown.