Acetylcholinesterase catalyzes the hydrolysis of acetylcholine. acetylcholine + H2O → acetate + choline The Km of this enzyme for acetylcholine is 90.0 μM. When [acetylcholine] = 150. μM, the rate of reaction was 40.0 μmol·mL-1 sec-1.
a. What is Vmax for this enzyme?
B. Assuming [acetylcholinesterase] = 5.00 nmol·mL-1, calculate kcat for this enzyme.