Which of the following is TRUE in the experiment to determine the direction of protein synthesis?
O Proteins were separated via centrifugation to remove incomplete chains.
O Full-length undigested hemoglobin was run on a gel to determine radiolabeled leucine incorporation.
O After adding radiolabeled leucine, synthesis was allowed to continue for several hours to ensure all proteins completed synthesis.
O Incomplete chains were run on the gel to ensure N-terminal data was included.
O More radiolabeled leucine was found at the C-terminus.
O More radiolabeled leucine was found at the N-terminus.
O Proteins synthesis was found to be in the 5'→3' direction.
O Proteins synthesis was found to be in the N→C direction.
O Had the incomplete chains not been removed, there would be an equal distribution of radioactivity across the protein.