In an eight-year experiment, a population of e. Coli, begun from a single cell, was grown in low-glucose conditions. The culture was transferred to fresh growth medium every 24 hours. Periodically, samples were removed from each population and the ability to survive in low-glucose conditions was compared with the original (ancestral) e. Coli population. What was the purpose of this experiment?
1) To study the effects of low-glucose conditions on the survival of e. Coli
2) To compare the growth rates of different populations of e. Coli
3) To determine the optimal glucose concentration for e. Coli growth
4) To investigate the genetic changes in e. Coli over time