The free-energy change for the hydrolysis of ATP to ADP + Pi, under standard conditions (1 M concentration of both reactants and products) is -7.3 kcal/mol. However, in the cytoplasm of the cell, the free-energy change is about -13 kcal/mol. Based on this observation, What would you predict the free-energy change for the reverse reaction (formation of ATP from ADP and Pi) would be under cellular conditions?
1) Less than 7.3 kcal/mol
2) About 7.3 kcal/mol
3) About 13 kcal/mol
4) About 26 kcal/mol