60x / 125 Oiltis 20 / 01 / EN285 An Olympus objective lens is given below. Assume GFP is used in the sample and its spectra are the same as in the graph. 1) Calculate the resolution of the microscope. 2) Assume a microtubule filament of 50nm (width) x 1000nm (length) is imaged by the objective lens. What is the size of the image? If the camera pixel size is 10um, what is the size of the image in unit of pixel #s? 1.0 or 0.8 Fluorophore Excitation and Emission Spectra Steep Peak Spectral Excitation Tails Spectrum Maximum Wavelength Emission Spectrum Symmetrical Spectral Profiles FWHM Sloped Excitation Sloped Tail Emission Tail 0.6 Normalized Excitation and Emission 0.4 0.2 150 nm Spectral Width 0.0 400 450 500 550 Wavelength (Nanometers) 600 650 Figure 2 JAPAN UplanFLN