How is oxygen binding affected when the Val residue is mutated to isoleucine? A. Substitution of valine with isoleucine would increase the affinity of oxygen binding because the side chain of Ile decreases steric hindrance and increases the size of the binding site.
B. Substitution of valine with isoleucine would decrease the affinity of oxygen binding because the side chain of Ile increases steric hindrance and decreases the size of the binding site.
C. Substitution of valine with isoleucine would increase the affinity of oxygen binding because the side chain of Ile increases steric hindrance and decreases the size of the binding site.
D. Substitution of valine with isoleucine would decrease the affinity of oxygen binding because the side chain of Ile decreases steric hindrance and increases the size of the binding site.