step 1 says to always add water to the tube first, then add additional reagents. Why do you suppose this is the case?
What color wavelength does 590 nm represent? Why is this wavelength chosen and how does this wavelength correspond with the color of bromophenol blue? What subatomic particle is responsible for the absorbance at this wavelength?
How should the absorbance of the 1:50 dilution compare with the dilution at 1:100?
What is the extinction coefficient (or molar absorbtivity constant) for the bromophenol blue?
Part 1 is divided into two sections, A and B. Which section address the accuracy of the pipetted volumes? Which section addresses precision of the pipetted volumes? Which statistical quantity would you use to discuss precision? Which statistical quantity would you use to discuss accuracy?