Know the components of spectrophotometer and their functions. There are 4 basic components: light source, sample holder, a device to separate light into its component wavelengths and a detector After light is collected from the sample it is focused onto the entrance slit of a monochromator This separates the light by wavelength using a dispersing element â†’ optical grating Light is then focused onto a charge coupled device that is made up of thousands of individual detectors so the intensity of light at each wave ght is measured Single beam: radiation sourceâ†’ wavelength isolatorâ†’ sample/ reference cell holderâ†’ photoelectric detectorâ†’ readout device Power supply connected to radiation source, photoelectric detector and readout device Double beam: radiation sourceâ†’ wavelength isolatorâ†’ rotating mirror beamâ†’ sample cell holderâ†’ semi transparent mirrorâ†’ photoelectric detectorâ†’ readout device radiation sourceâ†’ wavelength isolatorâ†’ rotating mirror beamâ†’mirrorâ†’ reference cell holderâ†’ semi transparent mirrorâ†’ photoelectric detectorâ†’ readout device Power supply connected to radiation source, rotating mirror, photoelectric detector and readout device

Question

contestada

Respuesta :