if an individual eats a high-carbohydrate diet in excess of calories needed for metabolism, glucose levels in the blood will likely be high. when glucose levels are high, the is feedback-inhibited by a high energy charge in the cell, and a buildup of accumulates in the mitochondrial matrix. there is both metabolic and hormonal control of fatty acid synthesis, which is primarily controlled by the activity of acetyl-coa carboxylase. covalent modification is a type of hormonal control of acetyl-coa carboxylase activity, and if an individual is eating a high-carbohydrate diet in excess of metabolic need, levels will rise, resulting in an in acetyl-coa carboxylase activity.