Question #2
A team of biologists has discovered a new enzyme that aids in the anaerobic breakdown of cellulose in
the stomachs of certain species of termites. The biologists have many questions about the nature of the
new enzyme and want to study the range of conditions in which the enzyme is active.
What is a logical procedure for the biologists to follow in testing the enzyme?


A) Beginning at a temperature of 0°C, decrease the temperature of the solution in which the enzyme is
placed in 5°C increments to see when the enzyme denatures.
B) Beginning at an initial pH of 2, increase the pH of the solution in which the enzyme is placed in
increments of 0.5 pH units to see when the enzyme denatures.
C) Beginning with an initial cellulose concentration of zero, gradually increase the substrate
concentration in the solution until it is so high that it causes the enzyme to denature.
D) Beginning with an initial oxygen concentration of 100 parts per million (ppm), gradually decrease the oxygen concentration in the enzyme's solution until the enzyme denatures.